Understanding CJC-1295 Without DAC: A Deep Dive into Its Role in Research

Published on June 29, 2025 by M5 Research Peptides

At M5 Research Peptides, we’re dedicated to supplying researchers with high-purity, research-grade peptides to advance scientific discovery. One of the most studied peptides in our catalog is CJC-1295 without DAC, a synthetic growth hormone-releasing hormone (GHRH) analog that has gained significant interest in biochemical and physiological research. In this blog post, we’ll explore what CJC-1295 without DAC is, its mechanism of action, its research applications, and why it’s a valuable tool for scientists. Our peptides are intended strictly for laboratory research and not for human or veterinary use.

What is CJC-1295 Without DAC?

CJC-1295 without DAC, also known as Modified GRF (1-29), is a synthetic peptide designed to mimic the structure and function of naturally occurring growth hormone-releasing hormone (GHRH). GHRH is a 44-amino-acid peptide secreted by the hypothalamus, which stimulates the pituitary gland to release growth hormone (GH). CJC-1295 without DAC is a truncated version of GHRH, consisting of the first 29 amino acids, with specific modifications to enhance its stability and activity.

The term “without DAC” (Drug Affinity Complex) distinguishes this peptide from CJC-1295 with DAC, which has an extended half-life due to a lysine linker that binds to albumin in the bloodstream. CJC-1295 without DAC has a shorter half-life, typically around 10–30 minutes, making it a preferred choice for researchers studying short, pulsatile GH release patterns that closely mimic the body’s natural rhythms.

Key Features of CJC-1295 Without DAC

• Amino Acid Sequence: 29 amino acids with four substitutions to improve resistance to enzymatic degradation.

• Molecular Weight: Approximately 3,365 Da.

• Purity Standards: At M5 Research Peptides, our CJC-1295 without DAC is synthesized to ≥98% purity, verified by independent third-party testing.

• Appearance: White lyophilized powder, reconstituted with bacteriostatic water for research use.

• Solubility: Highly soluble in water or saline, ensuring ease of use in experimental settings.

Mechanism of Action

CJC-1295 without DAC binds to GHRH receptors on the anterior pituitary gland, stimulating the release of growth hormone in a dose-dependent manner. Unlike direct GH administration, which bypasses the body’s regulatory feedback loops, CJC-1295 without DAC amplifies the natural pulsatile secretion of GH. This makes it an ideal candidate for studying the hypothalamic-pituitary axis and its downstream effects on cellular growth, metabolism, and repair mechanisms.

The peptide’s short half-life results in transient spikes in GH levels, which researchers find valuable for investigating:

• The dynamics of GH secretion in response to GHRH analogs.

• The interplay between GH and insulin-like growth factor-1 (IGF-1), a key mediator of GH effects.

• Feedback mechanisms involving somatostatin, which inhibits GH release.

Due to its short-acting nature, CJC-1295 without DAC is often studied in combination with other peptides, such as Ipamorelin, a growth hormone secretagogue that synergistically enhances GH release without significantly increasing cortisol or prolactin levels.

Research Applications

CJC-1295 without DAC has been widely explored in preclinical and in vitro studies. Below are some of the primary research areas where this peptide is making an impact:

1. Growth Hormone Regulation

Researchers use CJC-1295 without DAC to study the physiological mechanisms governing GH secretion. By mimicking natural GHRH, the peptide allows scientists to investigate how pulsatile GH release influences tissue repair, muscle growth, and metabolic homeostasis in animal models or cell cultures.

2. Metabolic Studies

GH plays a critical role in lipid and carbohydrate metabolism. CJC-1295 without DAC is used to explore how transient GH elevations affect fat oxidation, glucose uptake, and insulin sensitivity in experimental settings. These studies provide insights into metabolic disorders and potential therapeutic targets.

3. Muscle and Tissue Repair

The peptide’s ability to stimulate GH release makes it a valuable tool for studying muscle hypertrophy, tissue regeneration, and wound healing. Researchers have investigated its effects on myoblast proliferation and collagen synthesis in vitro, shedding light on the molecular pathways involved in tissue repair.

4. Neuroendocrine Research

The hypothalamic-pituitary axis is a complex system that regulates numerous physiological processes. CJC-1295 without DAC is used to study how GHRH analogs influence neuroendocrine signaling, including the interaction between GH, IGF-1, and other hormones like cortisol or thyroid hormones.

5. Synergistic Peptide Combinations

CJC-1295 without DAC is frequently studied alongside growth hormone secretagogues (GHS) like Ipamorelin or GHRP-6. These combinations allow researchers to investigate synergistic effects on GH release, receptor sensitivity, and downstream signaling pathways, providing a more comprehensive understanding of peptide interactions.

Advantages of CJC-1295 Without DAC in Research

• Mimics Natural GH Pulses: Its short half-life closely replicates the body’s endogenous GH secretion patterns, making it ideal for physiological studies.

• High Specificity: Targets GHRH receptors with minimal off-target effects, reducing experimental variability.

• Versatility: Suitable for both in vitro and in vivo studies, with applications across multiple research disciplines.

• Stability: Chemical modifications enhance resistance to enzymatic breakdown, ensuring consistent results during experiments.

Considerations for Researchers

When working with CJC-1295 without DAC, researchers should keep the following in mind:

• Storage: Store lyophilized powder at -20°C to maintain stability. Reconstituted peptide should be used within 2–4 weeks when stored at 4°C.

• Dosing: Experimental dosages vary widely depending on the study design (e.g., 1–10 µg/kg in animal models). Always follow institutional guidelines and ethical protocols.

• Synergy: Combining with GHS peptides may amplify effects but requires careful calibration to avoid receptor desensitization.

• Regulatory Compliance: Ensure compliance with local regulations, as peptides are subject to strict oversight for research purposes.

Why Choose M5 Research Peptides?

At M5 Research Peptides, we prioritize quality and reliability for your research needs. Our CJC-1295 without DAC is:

• Tested for purity and identity by independent third-party laboratories.

• Shipped with detailed certificates of analysis (CoA) for transparency.

• Packaged in custom, tamper-evident vials to ensure product integrity.

We also provide comprehensive support, including detailed product documentation, reconstitution guides, and a responsive customer service team led by a licensed pharmacist. Our commitment to quality and compliance allows you to focus on your research with confidence.

Conclusion

CJC-1295 without DAC is a powerful tool for researchers studying growth hormone dynamics, metabolic pathways, tissue repair, and neuroendocrine signaling. Its ability to mimic natural GHRH activity, combined with its short half-life and high specificity, makes it a versatile peptide for a wide range of experimental applications. At M5 Research Peptides, we’re proud to supply this peptide with the highest standards of purity and reliability, empowering scientists to advance their research.

Disclaimer: CJC-1295 without DAC is sold strictly for laboratory research purposes and is not intended for human or veterinary use. Always handle peptides in accordance with safety and regulatory guidelines.

Ready to incorporate CJC-1295 without DAC into your research? Visit M5 Research Peptides to explore our catalog and place your order today. For any questions, contact our team at support@m5researchpeptides.com.